Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient

Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient

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Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma.Control and tumor specific induced pluripotent stem cells were click here reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system.The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining.

The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo.This in vitro cellular model click here can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma.